- Suits both tomography and single particle cryo-EM
- Works for other soft materials that may require cooling when imaging
- User friendly for specimen loading
- Unique mechanism for specimen unloading
- Extended field of view at high tilt up to 80° (in TEMs with cryo pole pieces)
- Resolution to 0.18 nm
- Low drift rate of 1.5 nm/minute
- Operating temperature below -175 °C
Herpes simplex virus type 1 (HSV-1) KOS C-capsid.The capsids are icosahedral, 1250 Å in diameter and the sample includes DNA-filled C-capsids, as well as empty A-capsids and B-capsids that retain some component of the scaffold structure used in their assembly. The background includes strands of double-stranded DNA (dsDNA) that has been lost from some capsids. Taken by Thermo Fisher Scientific TF20 TEM (magnification 50000x at 200 keV, electron dose of ~20-25 e-/Å2) using Fischione Instruments’ Model 2550 Cryo Transfer Tomography Holder at -178 °C. Sample prepared using Thermo Fisher Scientific Vitrobot Mk III on Quantifoil R2/1 grid. Image courtesy of Alexander Makhov, Jamie Huffman, Fred Homa, and James Conway, University of Pittsburgh School of Medicine. |
Simple specimen loading and transfer
The innovative design of the specimen tip makes loading and unloading of the specimen easy. To load a specimen, the holder is inserted into the cryo work station or the TEM to cool down. The holder dewar and the cryo work station are both filled with liquid nitrogen. The modular design of the cryo work station’s grid box allows you to reposition the reservoirs to enhance ease-of-use for both right- and left-handed operation.
一旦实现了低于-175℃的温度,试样从栅格箱装入支架尖端。支架接受3毫米直径的TEM样品栅格,该网格由持有人的一体式集成摇篮夹具固定。当夹持器尖端缩回时,夹具会自动关闭。可选地,摇篮夹可以用镊子或随附的样品工具关闭。即使在高倾斜角度,夹具的薄型也可以最大限度地提高样品可视性。
样品支架尖端缩回位于支架内的主动冷却的Cryo屏蔽内。该屏蔽在转移到显微镜或从显微镜期间防止任何大气水冷凝在样品上。
快速冷却,延长保持时间
The Cryo Transfer Tomography Holder rapidly reaches a working temperature of less than -175 °C in either the cryo work station or a TEM. Hold times in excess of 4 hours are readily obtained with the 200 ml dewar capacity.
The temperature controller connects directly to the specimen holder dewar and displays the holder tip temperature.